STANDARD OPERATING PROCEDURE Operating Procedure for Manifold Filtration Apparatus

	STANDARD OPERATING PROCEDURE
	Department: Quality Control	SOP No.: QC/MB/
Operating Procedure for Manifold Filtration Apparatus		Effective Date:
		Review Date:
		Page No.:

1.0            OBJECTIVE:

1.1                   To lay down a procedure for operation of Manifold Filtration Apparatus.

2.0            SCOPE:

2.1                   This procedure is applicable to Manifold Filtration Apparatus located in microbiology laboratory at Pharma Private Limited.

Model:

3.0            RESPONSIBILITY:

3.1                   Microbiologist: Responsible for operation of the instrument.

3.2                   Section In-charge: Responsible for review the procedure

3.3                   QC Head: Responsible for implementing the Procedure.

3.4                   QA Head: Overall compliance.     

4.0            PROCEDURE:

4.1                 Ensure that the instrument is clean before starting.

4.2                 Connect the manifold filtration flushing tube end to the suction flask with hose pipe.

4.3                 Connect the other end of the suction flask with vacuum pump through hose pipe.

4.4                   Place the sterile membrane filters on the sterile filters holder and fix to the filtration Funnel.

4.5                 Arrange the filtration funnel to the manifold filtration flushing tube.

4.6                   Pour required quantity of the sample in the filtration funnel and start the vacuum pump, filtration will start and filtrate will collect in the suction flask.

4.7                   After completion of filtration remove the filter from the filter holder and transfer to the sterile agar media plates.

4.8                 Take another sterile filtration funnel to filter the next sample.

4.9                   After completion of filtration of all samples switch off the vacuum pump mains and   remove the filtration funnels from flushing tube.

4.10             Cleaning:

4.10.1         Clean all parts with hot water by using smooth brush.

4.10.2         After cleaning rinse all the parts with Milli-Q water or purified water.

4.11             Precautions:

4.11.1         Do not use steel wool or abrasive materials for cleaning since it will causes scratches over the components.

4.11.2         Do not wipe components with dry paper or cloth since fibres and lint left on Surfaces can generate electrostatic forces that attract more dirt.

4.12             Before starting the filtration activity sanitize the manifold filtration flushing tube with 70% IPA and sterilize the filtration funnels and suction flask at 121°C for 15 minutes in an autoclave.

5.0            ABBREVIATIONS:

QC: Quality Control	QA: Quality Assurance
SOP: Standard Operating Procedure	IPA: Iso Propyl Alcohol
°C: Degree centigrade	%: Percentage